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Feature Filtering

Description

This module filters molecular features that do not fit specified cutoff settings.

It has been observed that under certain circumstances, a large part of molecular features can be attributed to instrument noise Samples 2023. Therefore, FERMO allows to filter certain molecular features from the analysis:

  • For a given range of relative signal height/intensity (0-1.0, relative to the feature with the highest hieght/intensity), molecular features that are not inside this range in at least one sample are discarded
  • For a given range of relative signal area (0-1.0, relative to the feature with the highest area), molecular features that are not inside this range in at least one sample are discarded

These filters can be combined with each other to yield certain slices of the original data.

Nota bene: by default, features with a relative area of less than 0.01 are filtered from the analysis.

Parameters

Key Possible Values Default
activate_module true, false true
filter_rel_int_range_min 0.0-1.0 0.0
filter_rel_int_range_max 0.0-1.0 1.0
filter_rel_area_range_min 0.01-1.0 0.0
filter_rel_area_range_max 0.0-1.0 1.0

Explanation

  • filter_rel_int_range_min: specifies the minimum value of relative intensity to retain in analysis
  • filter_rel_int_range_max: specifies the maximum value of relative intensity to retain in analysis
  • filter_rel_area_range_min: specifies the minimum value of relative area to retain in analysis
  • filter_rel_area_range_max: specifies the maximum value of relative area to retain in analysis